《植物生理学报》 2014, 50(12): 1759-1763

预处理和培养温度对陆稻游离小孢子愈伤组织诱导及分化的影响

郭桂梅^1,2,*, 何婷^1,2,*, 高润红^1,2, 徐红卫^1,2, 陈志伟^1,2, 黄亦辰^1,2,3, 杨沙沙^1,2,3, 刘成洪^1,2, 李颖波^1,2, 陆瑞菊^1,2, 黄剑华^1,2,**

¹上海市农业科学院生物技术研究所, 上海201106; ²上海市农业遗传育种重点实验室, 上海201106; ³上海海洋大学水产与生命学院, 上海201306

通信作者：郭桂梅；E-mail: sw1@saas.sh.cn；Tel: 021-62201032

摘　要：

以陆稻品种‘云2号’和‘云3号’为材料, 分别研究了花药预处理、游离小孢子预处理和小孢子初期培养温度对小孢子愈伤组织产量及绿苗形成数的影响。结果表明, 将游离前的小孢子花药在32 ℃下预处理2 d, 或将游离小孢子在32 ℃下预处理2 d, 均可以提高小孢子的愈伤组织产量; 将游离小孢子置于32 ℃培养2 d后转入25 ℃培养优于只置于25 ℃培养, 可以明显提高小孢子愈伤组织产量及绿苗形成数量。

关键词：陆稻; 小孢子; 温度; 愈伤组织产量; 绿苗产量

收稿：2014-10-15   修定：2014-12-03

资助：上海市种业发展项目[沪农科种字(2011)第1号]和上海市农业科学院学科建设专项(LY11)。 * 共同第一作者。

Effects of Pretreatments and Culture Temperature on Callus Induction and Differentiation in Upland Rice Microspore Culture

GUO Gui-Mei^1,2,*, HE Ting^1,2,*, GAO Run-Hong^1,2, XU Hong-Wei^1,2, CHEN Zhi-Wei^1,2, HUANG Yi-Chen^1,2,3, YANG Sha-Sha^1,2,3, LIU Cheng-Hong^1,2, LI Ying-Bo^1,2, LU Rui-Ju^1,2, HUANG Jian-Hua^1,2,**

¹Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; ²Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China; ³College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

Corresponding author: GUO Gui-Mei; E-mail: sw1@saas.sh.cn; Tel: 021-62201032

Abstract:

Two cultivars of upland rice were used to investigate the effects of pretreatments of anther or isolated microspores and the initial culture temperature on callus yield and number of green plantlets. The results showed that 2 d of 32 ℃ pretreatment on anthers or isolated microspores could improve the callus yield. Comparing to continuous culture under 25 ℃, 2 d initial culture under 32 ℃ prior to subculture under 25 ℃ could remarkably improve the yields of the callus and green plantlet.

Key words: upland rice; microspore; temperature; callus yield; green plantlet yield